Screening and analysis of DNA fragments that show promoter activities inThermus thermophilus
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چکیده
منابع مشابه
Screening and analysis of DNA fragments that show promoter activities in Thermus thermophilus.
We have constructed a Thermus promoter probe vector pPP11 containing the promoterless heat-stable kanamycin resistance (Kmr) gene. Total DNA of T. thermophilus HB27 was randomly sheared into 100-200 bp fragments and inserted into the EcoRI site of pPP11. About 1000 clones which showed Kmr were obtained, and they were classified into three groups according to their Kmr levels. The DNA sequences ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1995
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1995.tb07511.x